Proceedings of the National Academy of Sciences · 1990 · 228 citations · 26 references
Life SciencesCell BiologyMolecular BiologyAgingGene ExpressionSignal TransductionAge-related Physiological ChangesAging PhysiologyBiological AgingCellular AgingGenetics Of AgingHeat StressCellular Heat Shock ResponseFibroblast CultureHeat Shock FactorsHeat Shock Protein GenesHeat Shock ResponseHeat Shock Response GenesHeat-induced Gene ExpressionSkin-derived Cells
The effect of aging on the induction of heat shock protein 70 (HSP70)-encoding gene expression by elevated temperatures was studied in cultures of lung- or skin-derived fibroblasts from young (5 mo) and old (24 mo) male Wistar rats. Although the kinetics of the heat shock response were found to be similar in the two age groups, we observed lower levels of induction of HSP70 mRNA and HSP70 protein in confluent primary lung and skin fibroblast cultures derived from aged animals. Additional experiments with freshly excised lung tissue showed a similar age-related decline in the heat-induced expression of HSP70.
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George M. Church, W Gilbert · Proceedings of the National Academy of Sciences · 1984 · 8.2K citations · Full text