Concepedia

Differentiation of Porcine <i>Brachyspira</i> Species by a Novel <i>nox</i> PCR-Based Restriction Fragment Length Polymorphism Analysis

Judith Rohde, Anja Rothkamp, Gerald F. Gerlach

Journal of Clinical Microbiology · 2002 · 86 citations · 12 references

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Abstract

A novel PCR-based restriction fragment length polymorphism analysis of the Brachyspira nox gene was developed. The restriction patterns for Brachyspira hyodysenteriae, B. pilosicoli, B. intermedia, B. murdochii, and B. innocens were highly distinct with two restriction endonucleases only. The assay proved to be user-friendly and robust.

References

12

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