Proceedings of the National Academy of Sciences · 1974 · 54 citations · 20 references
Life SciencesBiochemistryMicrobiologyDna RepairEnzymologyRadiobiologyAtp HydrolysisBiological Effects Of RadiationCellular Radiation ResponseDna DamageDna Strand BreaksSingle-strand Break RepairDna Repair MechanismsDna Single-strand BreaksDna Strand Break RepairRadiation DamageRepair Of Uv-induced Dna DamageSingle-strand Dna BreaksUltraviolet Radiation DamageUltraviolet-induced Dna Damage
The effect of ATP on the first step of excision repair of ultraviolet damage in DNA has been studied using toluene-treated E. coli. During postirradiation incubation, five to six times more single-strand breaks are formed in DNA in the presence of exogenous ATP than in its absence. The ATP-dependent as well as the ATP-independent endonucleolytic activities appear to be catalyzed by the same enzyme since both activities are almost completely absent in uvrA and uvrB mutants. An ATP-dependent endonucleolytic activity has been detected in nonirradiated toluene-treated E. coli. It is concluded that ATP is required in vivo for either the incision step of repair or an enzymatic reaction preceding it.
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Sedimentation studies of the size and shape of DNA
F. William Studier · Journal of Molecular Biology · 1965 · 2.2K citations