Luminescent Saccharide Biosensor by Using Lanthanide‐Bound Lectin Labeled with Fluorescein

Abstract

A new luminescent biosensor for complicated glycoconjugates was engineered on the basis of a lanthanide-complexed sugar-binding protein (lectin) modified with a fluorophore. By using luminescence resonance energy transfer (LRET), ratiometric luminescent sensing can be carried out and successfully applied to a luminescent assay for an enzymatic trimming of a glycoprotein (see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2005/z500072_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.