Deep‐tank culture of blue‐green algae in H₂O and D₂O

Abstract

Abstract A 160‐liter stainless steel algal growth tank has been constructed and has been used essentially continuously for over three years. Filamentous and unicellular blue‐green algae as well as a photosynthetic bacterium have been cultured using both ordinary and heavy water (99.8 atom % 2 H). By using a recycling technique, yields as high as 25 g/liter of 2 H 2 O have been obtained.