Multiple gene disruptions by marker recycling with highly efficient gene-targeting background (ΔligD) in Aspergillus oryzae

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink · Molecular and General Genetics MGG · 1984 · 2.4K citations

Orotidine-5′-phosphate Decarboxylase Activity, Cellular Enzymology, Biochemistry +7

Genome sequencing and analysis of Aspergillus oryzae

Masayuki Machida, Kiyoshi Asai, Motoaki Sano et al. · Nature · 2005 · 1.2K citations · Full text

Genetics, Fungal Evolution, Fungal Biology +4

Highly efficient gene replacements in <i>Neurospora</i> strains deficient for nonhomologous end-joining

Yuuko Ninomiya, Keiichiro Suzuki, Chizu Ishii et al. · Proceedings of the National Academy of Sciences · 2004 · 603 citations · Full text

Development of a novel quadruple auxotrophic host transformation system by<i>argB</i>gene disruption using<i>adeA</i>gene and exploiting adenine auxotrophy in<i>Aspergillus oryzae</i>

Feng Jin, Jun-ichi Maruyama, Praveen R. Juvvadi et al. · FEMS Microbiology Letters · 2004 · 239 citations · Full text

Engineering, Fungal Cell Biology, Genetics +16

Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae

Tadashi Takahashi, Tsutomu Masuda, Yasuji Koyama · Molecular Genetics and Genomics · 2006 · 181 citations

Enhanced Gene, Fungal Cell Biology, Aspergillus Sojae +7