DNA CONTENT OF PURIFIED PREPARATIONS OF MOUSE PURKINJE NEURONS ISOLATED BY A VELOCITY SEDIMENTATION TECHNIQUE

Abstract

Abstract— The DNA content of mouse Purkinje neurons was investigated employing a biochemical approach. Material for the biochemical assay was provided by means of a sedimentation velocity separation technique which yields bulk quantities of well‐preserved Purkinje perikarya in a high degree of purity. The same amount of DNA/cell was recorded for mixed cerebellar cell somata (7·6 ± 0±2 pg/cell), as for the Purkinje perikarya enriched fractions (7±2 & 0·2 pg/cell). No evidence could be found for the existence of a tetraploid DNA complement in mouse Purkinje neurons despite indications to the contrary from a parallel cytophotometric study.