Overproduction of cellular and activated Ha-ras proteins by mutating a synthetic gene.

Abstract

A synthetic gene for c-Ha-ras (Val-12) has been modified by cassette mutagenesis using restriction sites, ClaI-BssHII, to encode c-Ha-ras Gly-12. Genes for c-Ha-ras (Leu-61 and Arg-61) have been synthesized by joining newly synthesized oligodeoxyribonucleotides containing appropriate codons, together with previously obtained synthetic fragments. These genes have been expressed in E. coli and the products (p21) isolated. The guanosine diphosphate binding properties and guanosine triphosphatase activities of these p21 derivatives were studied.