Effects of Mutant and Antisense RNA of Phospholamban on SR Ca ²⁺ -ATPase Activity and Cardiac Myocyte Contractility

Abstract

K3E/R14E is a dominant negative mutant of PLB that disrupts the structural integrity and function of the endogenous PLB and consequently enhances SERCA2 activity and myocyte contractility. In neonatal myocytes, the decrease in steady-state abundance of PLB by asPLB also leads to increased SERCA2 activity.