Biosynthesis of bisabolene by callus cultures of Andrographis paniculata

Abstract

Experiments with intact callus cultures of Andrographis paniculata and a derived cell-free system indicated that (a) the biosynthesised γ-bisabolene has the Z-configuration (3); (b) the biosynthetic intermediate is 2-cis, 6-trans-(1)- and not 2-cis,6-cis-(2)-farnesol pyrophosphate; (c) in paniculide B (5) the ring carbon derived from C-2 of mevalonate is anti to the side chain.