<b>RAPID AND SENSITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR MEASUREMENT OF HGF IN RAT AND HUMAN </b><b>TISSUES </b>

Abstract

Hepatocyte growth factor (HGF) has organotrophic functions for regeneration of the liver, kidney and lung, through mitogenic, motogenic, and morphogenic activities. HGF concentration increases in sera of patients with various liver, kindey, and lung diseases. We designed a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) to measure HGF in crude tissue extracts. Monoclonal antibodies to human HGF were prepared and a mono-