Photovoltaic Determination of Hydrogen Peroxide with A Biophotodiode

Abstract

Abstract A novel biosensor, biophotodiode, is proposed for the determination of substances of biochemical and clinical importance. The biophotodiode is a unique combination of a photodiode and matrix-bound peroxidase. Since the enzyme catalyzes the luminescent reaction of the luminol-H2O2 system, this assembly is effective on the determination of hydrogen peroxide. The photocurrent of the sensor is correlated with the hydrogen peroxide concentration ranging from 1mM to 10 mM. A biophotodiode for glucose is also constructed by assembling a glucose oxidase-peroxidase bienzyme membrane and a photodiode. Key Words: BiophotodiodeLuminescent DeterminationEnzyme PhotodiodeLuminescent Biosensor.