A New Method for the Determination of Serum Nucleotidase

Abstract

The inhibition of the Nucleotidase effect of bone alkaline phosphatase by phenyl disodium orthophosphate is described. The conditions for inhibition and the effect on the normal 5N range and the elevated 5N values in liver diseases and in cases of high osteoblastic activity are reported. Die Hemmung des Nucleotidase-Effekts von alkalischer Phosphatase aus Knochen durch Dinatrium-phenylorthophosphat wird be-schrieben. Die Bedingungen für die Hemmung sowie der Effekt auf S'-Nucleotidase im Normbereich als auch auf erhöhten Aktivitäten bei Lebererkrankungen und Fällen hoher Osteoblasten-Aktivität werden dargestellt. Since REIS (1) reported that human liver homogenate hydrolyses AMP at pH 7.5 more rapidly than other phosphoric esters, serum activity towards AMP at pH about 7.5 is usually considered to be the result of two enzyme systems, i. e. a 5-nucleotidase system having only affinity for 5-nucleotides and a non-specific phosphatase which is able to split AMP as well. The measured total activity towards AMP is commonly simply referred to as 5-nucleotidase (5N)1).