BMB Reports · 2010 · 15 citations · 22 references
Raw264.7 CellsNitric OxideImmunologyCell DeathOxidative StressInflammationProtein ExpressionCell RegulationSignaling PathwayAmp-activated Protein KinaseReceptor Tyrosine KinaseAmpk GeneCell SignalingLps-induced ExpressionMolecular SignalingBiochemistryMolecular PathwayPep-1-ampk InhibitsChronic InflammationPharmacologyCell BiologyProtein PhosphorylationAnti-inflammatorySignal TransductionNatural SciencesProtein EngineeringCellular BiochemistryMedicine
AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that plays a central role in cellular metabolic stress. Modulation of nitric oxide (NO) and cyclooxygenase-2 (COX-2) is considered a promising approach for the treatment of inflammation and neuronal diseases. In this study, the AMPK gene was fused in-frame with PEP-1 peptide in a bacterial expression vector to produce a PEP-1-AMPK fusion protein. Expressed and purified PEP-1-AMPK fusion proteins were transduced efficiently into macrophage Raw 264.7 cells in a time- and dose-dependent manner. Furthermore, transduced PEP-1-AMPK fusion protein markedly inhibited LPS-induced iNOS and COX-2 expression. These results suggest that the PEP-1-AMPK fusion protein can be used for the protein therapy of COX-2 and NO-related disorders such as inflammation and neuronal diseases. [BMB reports 2010; 43(1): 40-45].
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Effects of Cerebral Ischemia in Mice Deficient in Neuronal Nitric Oxide Synthase
Zhihong Huang, Paul L. Huang, Nariman Panahian et al. · Science · 1994 · 1.6K citations