AIDS · 1989 · 15 citations · 0 references
Life SciencesImmunologyMolecular BiologyEnzymologyMolecular VirologyVirologyAntiviral ImmunityInterferon ResponsesViral InfectionsViral PathogenesisViral ReplicationViral Rna BiologyAntiviral Interferon ResponsesAntiviral Mechanisms Of ActionInhibition Of Interferon ResponsesInterferon ProductionInterferon Response To Viral InfectionRibonuclease MechanismsSindbis Virus InfectionViral Replication Inhibitors
In this study the effect of interferon (IFN) on uninfected cells (H9-) and cells infected (H9+) with HIV-1 was investigated. In both H9- and H9+, IFN was unable to inhibit Sindbis virus replication. HIV-1 replication was inhibited by a maximum of 22% at 1000 U/ml of IFN. In both H9- and H9+ cells IFN exhibited a limited antiproliferative effect. There was activation of the 2'-5' oligo-A-synthetase (E enzyme) and the protein kinase enzyme systems, but low activation of the ribonuclease F was seen in both H9- and H9+ cells. From these results it can be concluded that IFN produces a limited antiviral and antiproliferative effect in both H9- and H9+ cells, and a defective ribonuclease F pathway might be responsible for this limited activity.