RESPIRATION BY CULTIVATED ASTROCYTES AND NEURONS FROM THE CEREBRAL HEMISPHERES

Abstract

Abstract ‐Rates of oxygen uptake were measured in chick and/or rat astrocytes and neuronal cells cultivated for 2–4 weeks in Falcon flasks or Rose chambers. All the preparations were found to have respiratory rates between 0.4 and 0.8 × 10 −5 μl/h O 2 per cell. Based upon measurements of cell diameters these values were recalculated to about 570 μmol/g wet wt. for the neuronal cells and 130 μmol/g wet wt. for the glial cells. The results are compared with previous data of oxygen uptake by neurons and glial cells separated by other procedures.