Solid-State Deuterium NMR Studies Reveal μs-ns Motions in the HIV-1 Transactivation Response RNA Recognition Site

Abstract

Solution and solid-state NMR measurements were used together to examine motion in three sites in the HIV-1 TAR RNA. We wished to investigate the dynamics facilitating the conformational rearrangements the TAR RNA must undergo for Tat binding and in particular to characterize the full range of motional time scales accessible to this RNA. Our results demonstrate that the dynamics in TAR involving residues essential to Tat binding include not only the faster motions detected by solution relaxation measurements but also a significant component in the μs-ns time scale.