Purification of <i>Eimeria</i> Sporozoites by DE‐52 Anion Exchange Chromatography

Abstract

An anion exchange column of DE-52 has been used to purify Eimeria sporozoites from a post- excystation mixture of oocysts, oocyst shells, sporocysts, sporocyst shells, and sporozoites. The mean recovery from several experiments was 94% and virtually all non-sporozoite material was removed. Infectivity studies in vitro with sporozoites showed that they were viable after purification and were at least as infectious as the unpurified sporozoites; furthermore, oocysts in the crude preparation could be recovered from the DE-52 cellulose by resuspending them in a 20% (w/v) sodium chloride solution.