Catalytic hydrolysis of cAMP

Abstract

[Co(trien)(OH 2 )(OH)]Cl 2 is highly efficient in catalyzing the hydrolysis of cAMP to adenosine in neutral water (6 × 10 −6 M −1 s −1 at 50 °C). The cobalt complex promoted hydrolysis of cAMP is estimated to be about a hundred million times faster than the uncatalyzed hydrolysis of cAMP. In the time that [Co(trien)(OH 2 )(OH)]Cl 2 produces 50% cleavage of cAMP, [Co(en) 2 (NH 3 )(OH)]Cl 2 or ZnCl 2 has no observable effect on the hydrolysis of cAMP. A detailed mechanism is presented for the cobalt complex promoted hydrolysis of the phosphodiester.