A COMPARISON OF AGAR AND ACRYLAMIDE GEL MICROIMMUNOELECTROPHORETIC TECHNIQUES FOR THE SEPARATION OF URINARY COLLOIDS*

Abstract

Summary The differences in the immunoreaction in both gels are small, especially if one knows their origin. Polyacrylamide has the general advantage of being technically easier to handle and quicker than agar gel. The minor expenditure of working time, the shorter running time, the higher elasticity, especially preferable for solid samples, overcome the disadvantage of the longer time required for the immunoreaction. And it is especially important and useful that with pqlyacrylamide gel you can examine several protein solutions simultaneously on one plate under equivalent conditions. This is very convenient for investigation of optimal antigen‐antibody concentrations and for testing of unknown antisera.