Effects of chondroitinase ABC on degenerative intervertebral discs.

Abstract

The effects of chondroitinase ABC on surgically induced degenerative rabbit intervertebral discs were determined during a 12-week period by magnetic resonance imaging, radiography, and histologic examination. Rabbit intervertebral discs were surgically extruded, inducing disc degeneration 12 weeks before injection of chondroitinase ABC. Magnetic resonance imaging showed a hypointense area in the center of the surgically induced disc degeneration. After injection of chondroitinase ABC, the hypointense area became more intense, but reversed somewhat by the end of the 12-week period. Additional evidence of the effects of chondroitinase ABC on a surgically induced degenerative disc model was shown by radiographic evidence of shrinkage of the disc after injection. Histologic examination revealed a fibrous degenerative disc induced by surgical extrusion. However, the staining properties of the matrix of the nucleus pulposus was similar to that of normal discs before chondroitinase ABC injection, but diminished after the injection, with slight recovery at 12 weeks. The results suggest that chondroitinase ABC induces chemonucleolysis in the degenerated disc. Also, chondroitinase ABC does not destroy the degenerative disc matrix ability to regenerate after 12 weeks.