Science · 1980 · 112 citations · 22 references
Life SciencesBiochemistryImmunologyCellular ImmunologyImmunotechnologyMonoclonal AntibodiesAntibody CharacterizationAntibody PurificationHybridoma TechnologyMonoclonal Antibody TechnologyTherapeutic Antibody DevelopmentTherapeutic Monoclonal AntibodiesHybridoma Cell Line DevelopmentHybridoma Cell LinesHybridoma FormationHybridoma-derived AntibodiesImmunoglobulin GlycosylationImmunoglobulin ProductionMyeloma Cell LinesStable Producer Cell Lines
Hybrid cells formed between human lymphocytes and mouse myeloma cells produce human immunoglobulin in culture. Stable antibody-producing cell lines can be isolated after multiple cycles of low-density passage, cloning, and continued selection for immunoglobulin production. The origin and characteristics of a hybrid of human and mouse cells is described. This hybrid produces high concentrations (8.3 micrograms per milliliter) of human immunoglobulin M reactive with the terminal disaccharide of the Forssman glycolipid. These findings point to the potential use of human-mouse hybrid cells as a source of human monoclonal antibodies for therapeutic and diagnostic purposes.
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Continuous cultures of fused cells secreting antibody of predefined specificity
Georges Köhler, César Milstein · Nature · 1975 · 17.2K citations
Pharmacy, Cell Biology, Immunology +11
Antibodies to major histocompatibility antigens produced by hybrid cell lines
G. Galfré, Sebastian Howe, César Milstein et al. · Nature · 1977 · 1.7K citations