A cell line from a human salivary gland mixed tumor

Abstract

A cell line called Nagoya-78 has been established from a human salivary gland mixed tumor. The cytoplasm of the cells contain much glycogen but little acid polysaccharide and mucin. The ultrastructure of the cells exhibit epithelial characteristics with desmosomes, and no virus particles are present. The doubling time is around 24 hours. The cell line can be cultured as a suspension, but the growth rate is lower than that of stationary cultures. The chromosome constitution approximates trisomy with 5 marker chromosomes. The cells grow slowly in the cheek pouch of unconditioned hamsters.