Sequence analysis and expression of a cDNA clone encoding a 98‐kDa allergen in <i>Dermatophagoides farinae</i>

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.4K citations · Full text

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

John M. Chirgwin, Alan Przybyla, Raymond J. MacDonald et al. · Biochemistry · 1979 · 22.2K citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Protein Expression, Biochemistry, Single-step Purification +10

[2] New M13 vectors for cloning

Joachim Messing · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 5.4K citations

Molecular Biology, Genetic Engineering, Gene Vector +2