Extraction and Quantitative Characteristics of Actomyosin in Canine Heart Muscle

Abstract

The conditions for quantitative extraction of actomyosin from dog heart ventricular muscle were studied. By use of appropriate techniques and conditions, yields of actomyosin comparable to those found by others for a variety of muscles were obtained. The method used could be standardized to give reproducible values with both fresh and frozen muscle. Actomyosin so obtained retained its characteristic properties as evidenced by its viscosity response to ATP and its enzymatic activity.