Optimization of transport processes in etched track-based biosensors

Abstract

A diffusion calculation was performed to simulate the migration kinetics of both analytes and enzymatic reaction products in enzyme-clad nanopores of different sizes and shapes, to estimate the limits of detection, response time and cleaning time of such biosensors, via the maximum possible reaction product enrichment in such pores and the time required for saturation of the corresponding concentration distributions. From this, conclusions concerning the optimum biosensor dimensions are derived.