Journal of the American Society for Mass Spectrometry · 1997 · 95 citations · 19 references
PharmacyBiochemistryPharmaceutical AnalysisAnalytical ChemistryChromatographic AnalysisMass Spectrometric AnalysisChromatography CouplingIonization TechniquesMass SpectrometryPeptide AnalysisPeptide IdentificationProtein SeparationCapillary ElectrophoresisCapillary Zone ElectrophoresisMass Spectrometric DetectionMass Spectrometry ProteomicsPeptide Mass SpectrometryPeptide SeparationRibonuclease Chemistry
Peptide standards and tryptic digests of ribonuclease B are separated by comprehensive two-dimensional reversed phase liquid chromatography (RPLC) and capillary zone electrophoresis (CZE) and detected on-line by electrospray mass spectrometry. The RPLC column is coupled to the CZE column by a transverse flow gating interface. A new rugged microelectrospray needle is described that combines high ionization efficiency, low flow rates, and a sheath flow. The result is a system combining the separation capabilities of both RPLC and CZE with on-line mass spectrometric detection, all in about 15 min.
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