Genetic comparison between Pinus sylvestris and P. mugo using isozymes and chloroplast DNA

Abstract

Pinus sylvestris and P. mugo populations from Poland and Czechoslovakia were compared using genetic variability at isozyme markers, chloroplast DNA variation, and mating system measurements. Two isozyme loci were found to differ between the species. P. mugo was as variable at isozyme loci as P. sylvestris. Diagnostic cpDNA fragments were found using the restriction enzyme Bcl‐I. Populations that were morphologically classified as hybrids were found to be pure species, based both on isozyme and cpDNA results.