The Journal of Pathology · 1997 · 114 citations · 10 references
Immunocytochemical TechniqueEngineeringImmunologyTyramine AmplificationSensitive Detection MethodBiosensing SystemsBioanalysisImmunochemistryAnalytical ChemistryAntibody EngineeringClinical ChemistryAnalytical BiotechnologyImmunohistochemistryBiochemistryAntibody ScreeningPharmacologyBiomolecular EngineeringBiotinylated TyramineLight Microscopic ImmunohistochemistryBioelectronicsBiotechnologyProtein EngineeringChemical ProbeMedicine
The study introduces a highly sensitive light‑microscopic immunohistochemistry method. It achieves greater sensitivity by horseradish peroxidase–catalyzed deposition of biotinylated tyramine at immunoreactive sites, then detecting biotin with a streptavidin–biotin–horseradish peroxidase complex. The technique is broadly applicable, converting previously non‑reactive antibodies into strong, reproducible signals even in antigen‑retrieval formalin‑fixed, paraffin‑embedded sections, and permits many antibodies to be used at higher dilutions.
A highly sensitive method for light microscopic immunohistochemistry is described. The increased sensitivity compared with current methodologies is based on the horseradish peroxidase-catalysed deposition of biotinylated tyramine at the sites of immunoreactivity, followed by the detection of the biotin with streptavidin biotin horseradish peroxidase complex. This method is of general applicability in immunohistochemistry and has several important advantages over currently used immunohistochemical detection procedures. The most significant advantage is that several antibodies which to data have been non-reactive, even in antigen-retrieval formalin-fixed, was-embedded sections, now show strong and reproducible immunoreactivity using biotinylated tyramine amplification. In addition, many other antibodies can be used at significantly higher dilutions.
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Antigen unmasking on formalin‐fixed, paraffin‐embedded tissue sections
Giorgio Cattoretti, Stefano Pileri, Carlo Parravicini et al. · The Journal of Pathology · 1993 · 725 citations