Controlled transgene expression by E1‐E4‐defective adenovirus vectors harbouring a “tet‐on” switch system

Abstract

In the recombinant adenovirus context, the CMV* but not the TK* promoter is sensitive to transcriptional interference resulting in high doxycycline-independent expression. By paying attention to the rtTA expression, moderate and high induction can be obtained in vivo and in vitro accordingly.