DETERMINATION OF ACETAMIPRID BY HPLC-FLUORESCENCE WITH POST-COLUMN PHOTODERIVATIZATION AND HPLC-MASS SELECTIVE DETECTION

Abstract

ABSTRACT Two methods are described for determining the highly polar pesticide acetamiprid in vegetables. Residues are extracted with ethyl acetate and coextratives are removed with a graphitised Carbon-based packing ENVI-carb cartridge. Analysis is performed with goods results, by HPLC with post-column photoderivatization, and fluorescence detection and by HPLC-Electrospray ionisation mass spectrometry. The clean-up step is not necessary in this last case. LODs were 6 μg L−1 for HPLC-fluorescence detection using matrix matched standards and 1.5 μg L−1 for HPLC-ES-MS detection. Recovery data were obtained by fortifying vegetable matrix at 0.01, 0.1, and 0.5 mg kg−1, with recoveries between 65 and 75% for the HPLC-fluorescence and between 72 and 77% for HPLC-ES-MS.