Analytical Letters · 1977 · 70 citations · 3 references
EngineeringBiochemistryMicrobiologyBiosensorsMicrobial PhysiologySensorsBacterial PhysiologyElectrochemical BiosensorsElectrochemical SensorsEnzyme-based BiosensorsGas SensorsMicrobial BiosensorsBacterial BiosensorsBacterial SensorsElectrode InterfacesMicrobial Electrochemical TechnologiesPotentiometric BiosensorsPotentiometric DetectionPotentiometric SensorsRegenerable Membranes
Abstract The use of living bacteria held at the surface of an NH3 gas sensing membrane electrode permits the construction of a potentiometric sensor for L-aspartate. It is shown that the bacterial electrode, when stored in nutrient media, can have an appreciably longer lifetime than the corresponding electrode based on isolated L-aspartase enzyme. The response range and dynamic properties of the bacterial electrode are comparable to those of the enzyme electrode.
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