A Simple Method for the Removal of Rabbit Corneal Epithelium Utilizing n-Heptanol

Abstract

A new method for removing corneal epithelial cells, using n-heptanol, is faster, more precise, and less damaging than scraping, which is the usual method of removing surface epithelium. We compared the results of n-heptanol removal with those of epithelial scraping, using scanning and transmission electron microscopy. Heptanol, unlike scraping, does not damage either the basement membrane or the extracellular stromal components. The rate of healing of corneal epithelium after heptanol treatment is similar to published observations of scraped corneas. Both scraping and heptanol destroy keratocytes, which underlie the basement membrane.