STUDIES IN FOREST PATHOLOGY: VI. IDENTIFICATION OF CULTURES OF WOOD-ROTTING FUNGI

Mildred K. Nobles

Canadian Journal of Research/Canadian journal of research · 1948 · 262 citations · 0 references

Concepts

TL;DR

The study compiles a list of 126 wood‑rotting fungi, many of the most frequently encountered species in decay research, selected without regard to host or locality. The authors present a numerical key and accompanying descriptions to identify cultures of these species and allow future species to be incorporated. Cultures were grown on malt agar (with or without gallic/tannic acid) in Petri dishes, incubated in the dark at room temperature, and examined weekly for six weeks, with the key based on macroscopic and microscopic cultural characters such as pigmentation, agar color changes, growth rate, hyphal type, spore formation, cystidia, setae, and fruit‑body development. The diagnostic value of host relationships and physiological and morphological characters was confirmed, enabling species identification.

Abstract

A numerical key for the identification of cultures of 126 species of wood-rotting fungi, with accompanying descriptions of the cultural characters of each species, is presented. The list of species includes many of the fungi most frequently encountered in studies of decay in various hosts, the species having been chosen without restriction as to host or locality. The descriptions and key have been based on cultural characters, both macroscopic and microscopic, that can be demonstrated readily without the aid of elaborate equipment or methods. Thus, cultures were grown in Petri dishes on Difco malt agar and on malt agar to which gallic or tannic acid had been added. They were incubated in the dark at room temperature and examined at weekly intervals for six weeks. Host relationships and physiological and morphological characters proved to be of diagnostic value. The physiological characters used in the key include the pigmentation of the mycelial mat, color changes induced in the agar by the fungus, rate of growth, and effect on media containing gallic or tannic acid. Morphological characters used in diagnosis were types and septation of hyphae, occurrence of secondary spores – chlamydospores, conidia, oidia – and of special structures, such as cystidia and setae, and the formation of fruit bodies. The numerical key will permit the interpolation of additional species as they are described and their code numbers prepared.