Journal of Applied Ichthyology · 2003 · 14 citations · 11 references
Life SciencesGeneticsMolecular BiologyDiagnostic MicrobiologyMolecular VirologyVirologyAnimal VirusesDna Virus BiologyIn Situ HybridizationViral GeneticsViral GenomicsViral InfectionsDna Fragment DetectionHerpesviruses In WildlifeNucleic Acid Hybridization AssaysNucleic Acid ProbesRecombinant Dna ConstructionRestriction Enzyme DigestionViral Genome DetectionViral Isolation
A nucleic acid probe for the Herpesvirus anguillae (HVA) Taiwan isolate was constructed using recombinant DNA techniques. This probe consisted of a specific viral DNA fragment (1550 bp) generated by digestion of HVA DNA with the restriction enzyme HindIII, and labeled non-radioactively with digoxigenin (DIG). The probe was used to detect the HVA genome from HVA-infected cell cultures and tissue specimens prepared from infected eels, using either dot blot or in situ hybridizations.
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