Endothelial cells analysis with the TOPCON specular microscope SP-2000P and IMAGEnet system

Abstract

We suggest the use of the clearest image with the retraced method for endothelial cell analysis with the IMAGEnet system, as the variability in hexagonality results between the first and the clearest images can be clinically significant. For the same set of images captured by one examiner, the TOPCON IMAGEnet system was reliable in determining ECD and ACS, and was fairly reliable in determining hexagonality and CV. When images were captured on different days or by different examiners, the TOPCON SP-2000P with the IMAGEnet system gives repeatable and reproducible values for ECD and ACS only. Caution should therefore be exercised when using CV and hexagonality to monitor endothelial changes in comparative studies.