Ultrasensitive colorimetric detection of protein by aptamer–Au nanoparticles conjugates based on a dot-blot assay

Abstract

A simple, rapid and ultrasensitive colorimetric detection of protein using aptamer-Au nanoparticles (AuNPs) conjugates based on a dot-blot array has been developed, which was combined with the unique optical properties of AuNPs, enabling the visual detection of protein within minutes without any instrument.