A millipore filter assay for antibodies to native dna in sera of patients with systemic lupus erythematosus

Abstract

Abstract Serum levels of antibody to native DNA (nDNA) in patients with systemic lupus erythematosus (SLE) correlate with disease activity. We describe a sensitive, simple and rapid assay for these antibodies based on the observation that 3 H‐labeled nDNA passes through Millipore filters while 3 H‐nDNA complexed with specific antibody is retained. The simplicity and rapidity of this assay should allow general utilization of serum anti‐nDNA antibody determinations for monitoring disease activity in patients with SLE.