Bacteriological and Molecular Description of Brucella Species Isolated from Milk and Vaginal Swab Samples of Aborted Cattle in Kars Region

Abstract

Summary In this study, the milk and vaginal swab samples, collected from each of 250 cows raised in Kars and Selim areas, were analysed for culturing Brucella species. These animals had a history of abortion and they were unvaccinated against Brucellosis. Based on the bacteriological examination, Brucella spp. was isolated from total of 27 (%5.4) samples, 11 (%4.4) and 16 (%6.4) originated from milk and vaginal swab samples, respectively. These isolates were found to be Brucella spp. Are field isolates based on the PCR analysis using primers specific to Brucella genus (Omp bp26) and erythritol (ery). All of the isolated strains were typed as B. abortus biotype 3. RAPD-PCR analysis showed that DNA patterns of these isolates were found to be similar to that of B. abortus S19.