Highly sensitive detection of saccharides under physiological conditions with click synthesized boronic acid-oligomer fluorophores

Abstract

Two phenylboronic acid based saccharide sensors bearing conjugated oligomer fluorophores with linear and cruciform π-frameworks were synthesized in a modular approach utilizing a Cu-catalyzed alkyne azide cycloaddition (click) reaction. The cruciform fluorophore showed excellent saccharide sensing function under physiological conditions in the mM range, whereas the linear fluorophore gave very limited sensing functions. The different fluorescent sensing behaviours highlight the important role of oligomer fluorophore in the development of effective saccharide sensors.