Cibacron Blue F3GA incorporated magnetic poly(2‐hydroxyethyl methacrylate) beads for lysozyme adsorption

Abstract

Abstract Lysozyme adsorption onto Cibacron Blue F3GA incorporated magnetic poly(2‐hydroxyethyl methacrylate) (mag‐PHEMA) beads was investigated. Mag‐PHEMA beads (80–120 μm) were produced by dispersion polymerization. The triazine dye Cibacron Blue F3GA was incorporated covalently as a dye ligand. These dye‐incorporated beads, having a swelling ratio of 34% (w/w) and carrying different amounts of Cibacron Blue F3GA (12.2–42.8 μmol/g) were used in lysozyme adsorption studies. The effects of the initial concentration, pH, ionic strength, and temperature on the adsorption efficiency of dye‐incorporated beads were studied in a batch reactor. The effects of the Cibacron Blue F3GA loading on the lysozyme adsorption were also studied. The nonspecific adsorption of lysozyme on the mag‐PHEMA beads was 0.8 mg/g. Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 295 mg/g. More than 90% of the adsorbed lysozyme was desorbed in 1 h in a desorption medium containing 1.0 M KSCN at pH 8.0. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 719–725, 2004