FEBS Letters · 1987 · 13 citations · 23 references
Life SciencesBiochemistryEnzymologyPlant BiochemistryProtein BiochemistryCatalytic MechanismsEnzyme CharacterizationEnzyme StabilityRedox EnzymesRedox ProcessesEnzymesIonic StrengthIonic Strength DependenceOxidoreductase ActivityOxidoreductase Enzymes
Spinach sulfite reductase has been shown to co‐migrate during gel filtration chromatography at low ionic strength with spinach ferredoxin. No co‐migration was observed at high ionic strength. These results indicate that the two proteins form a high‐affinity, electrostatically stabilized complex, as had previously been demonstrated for three other ferredoxin‐dependent, plant enzymes. Modification of 3–4 ferredoxin carboxyl groups had little detectable effect on the ferredoxin‐sulfite reductase interaction.
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