Presence of One Polypeptide Chain in Valyl and Isoleucyl tRNA Synthetases from <i>Escherichia coli</i>

Abstract

Determination of the molecular weight of valyl and isoleucyl tRNA synthetases under denaturing conditions leads to the conclusion that both enzymes are made of a single polypeptide chain of a molecular weight of 110000 approximately. The presence of one amino acid binding site per chain was determined by equilibrium dialysis.