Physica status solidi. C, Conferences and critical reviews/Physica status solidi. C, Current topics in solid state physics · 2010 · 10 citations · 0 references
EngineeringBioengineeringMaterials ScienceBiomaterialsBiosensorsSensorsMicroscale BiosensorsNanomaterial-based BiosensorsAntibodiesAntibody ImmobilizationBovine Serum AlbuminFunctionalizationMatrix MetalloproteinasesPorous SiliconSensingSensor Functionalization
Abstract Porous silicon microcavity (PSiMc) structures were used as support material for specific sensing of matrix metalloproteinases (MMPs). For lower concentrations of MMP‐8, the structures were tested with two types of functionalization methods. Silanization of the oxidized porous silicon structures, followed by glutaraldehyde chemistry was found to give very inconsistent results. The use of biotinilated bovin serum albumin linked to the naked PSiMc was found to be an alternative method to attach the anti MMP‐8 human antibody, previously modified with streptavidin, which was further used to sense MMP‐8 (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)