Tissue fractionation studies. 18. Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients

Abstract

Beaufay, Bendall, Baudhuin, Wattiaux & de Duve (1959) described experiments in which mito- chondrial fractions from rat liver were partly re- solved into their components by density equilibra- tion in sucrose gradients, with either H20 or D20 as solvent. The results obtained in these investigations provided additional confirmation of the existence of lysosomes as a separate group of particles, distinct from mitochondria, and sug- gested further that urate oxidase, though almost.