Molecular Microbiology · 1998 · 292 citations · 40 references
AgricultureMicrobiologyMolecular BiologyHost-pathogen InteractionsMicrobial PathogenesisPlant-microbe InteractionsBacterial Pathogenesis In PlantsBacterial Plant PathogensBacterial Secretion SystemsPlant PathogenesisPlant-pathogen InteractionsVirulence Gene RegulationLegume-rhizobium SymbiosisMolecular Basis Of VirulenceNodulation GenesPathogenesis MechanismsPathogenesis Of Plant-associated BacteriaPathogenicity MechanismsPlant-associated Bacterial SecretionRhizobium Symbiosis
The symbiotic plasmid of Rhizobium sp. NGR234 carries a cluster of genes that encodes components of a bacterial type III secretion system (TTSS). In both animal and plant pathogens, the TTSS is an essential component of pathogenicity. Here, we show that secretion of at least two proteins (y4xL and NolX) is controlled by the TTSS of NGR234 and occurs after the induction with flavonoids. Polar mutations in two TTSS genes, rhcN and the nod-box controlled regulator of transcription y4xl, block the secretion of both proteins and strongly affect the ability of NGR234 to nodulate a variety of tropical legumes including Pachyrhizus tuberosus and Tephrosia vogelii.
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Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.3K citations
R Factor Transfer in Rhizobium leguminosarum
J. E. Beringer · Microbiology · 1974 · 2.2K citations
In vitro insertional mutagenesis with a selectable DNA fragment
Pierre Prentki, Henry Krisch · Gene · 1984 · 1.7K citations