Applied Organometallic Chemistry · 2002 · 12 citations · 15 references
Natural SciencesChemistryToxicologyEnvironmental ChemistryEnvironmental ToxicologyPesticide ToxicologyHerbicide Analytical ChemistryHerbicide Residue ChemistryHerbicide ToxicologyPesticide ChemistryPesticide EcotoxicologyPesticide Residue AnalysisAnalytical Detection Of Organic PollutantsAnalytical Methods DevelopmentAssay SensitivityEnvironmental Monitoring Of HerbicidesImmunoassays For HerbicidesPesticide DetectionPhenylurea HerbicidesStructure Of Metal Carbonyl Complexes
Abstract We report a new, non‐isotopic, immunoassay (CMIA) for chlortoluron, a pesticide of the phenylurea family. The novel feature of this assay is the use of metal carbonyl complexes as tracers and Fourier‐transform infrared (FT‐IR) spectroscopy as the detection method. We describe the synthesis of three tracers, derivatives of chlortoluron with an attached cyclopentadienyl manganese tricarbonyl moiety. Quantitative analysis of these tracers, by FT‐IR spectroscopy was performed by simple measurement of the absorbance peak at 2033 cm −1 , characteristic of the ν CO of the metal‐carbonyl unit. Immunoassays were carried out using the IgG fraction of rabbit antiserum. Standard curves obtained by CMIA for the three tracers showed the feasibility of this technique for chlortoluron assay. However, the IC 50 and IC 90 values obtained with the three tracers varied widely, with the long‐chain tracer 12 giving the most sensitive assay for chlortoluron, with an IC 50 value of 50 pmol (21 µg l −1 ) and an IC 90 of 6.5 pmol (2.7 µg l −1 ). Copyright © 2002 John Wiley & Sons, Ltd.
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