Effects of high energy dry milling on biphase calcium phosphates

Abstract

AbstractThe effect of high energy dry milling on the structural and crystalline state of sintered biphase calcium phosphates was studied. After various periods of grinding, the initial biphase calcium phosphate material alters its crystalline structure and phase composition. The phase transformations achieved during milling were recorded by powder X-ray diffraction, scanning electron microscopy, infrared spectroscopy and chemical analysis. X-ray diffraction analysis of samples milled for 20 h showed that the initial composition of the biphase ceramics changed and part of β-tertiary calcium phosphate was nanocrystalline and partially in amorphous state. Hydroxylapatite fully transformed into nanocrystalline phase. Cytotoxicity tests of samples milled for 20 h clearly present cellular viability with pronounced biological activity.