Organic & Biomolecular Chemistry · 2003 · 35 citations · 15 references
Life SciencesBiochemistryMicrobiologyBacteriologyBiocatalysisEnzymologyMicrobial BiocatalysisMicrobial BiosensorsMicrobial BiotransformationBaeyer-villiger OxidationBiocatalyst Activity AssaysEnzyme-based AssaysFluorogenic Enzyme SubstratesFluorometric Enzyme AssaysMicrobial Culture TestingMicrobial Enzyme FunctionMicrobial Extracellular Enzymes
The first fluorogenic assay allowing for detection of microbial enzymes able to perform Baeyer-Villiger oxidation is described. This is based on the use of 4-oxopentyl umbelliferyl ether 1 as a fluorogenic substrate. When Baeyer-Villigerases active against this test ketone are present in the selected whole cells, 1 is transformed into 3-hydroxypropyl umbelliferyl ether 3, which, in a subsequent step, releases the fluorescent product umbelliferone. Different microorganisms, known to be endowed with Baeyer-Villigerase activity, were assayed.
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