Science · 1992 · 707 citations · 28 references
Life SciencesMolecular BiologyPlant BiologyBiological Transport PhenomenaPlant BiochemistryPlant Molecular BiologyChannel StructureIon Channel ExpressionIon Channel PhysiologyIon ChannelsMembrane TransportTransport Across Biological MembranesAmino AcidsAnkyrin Repeat DomainsCellular Potassium UptakeChannelsIon Transport ProteinsPotassium Channel FunctionPotassium Channel GenesPotassium Transport Proteins
A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.
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